According to financial reports, people in West Bengal has a higher tendency of neglecting microfinance loan, which increases the amount of Non-performing Asset (NPA) in the Microfinance Companies (MFC). This paper is aimed to check the impact of NPA on profitability of microfinance companies in India from the angle of four selected microfinance companies. This study is performed with secondary data collected for the period of 2018-2023 from money control website and the considered companies are, Bandhan Bank Ltd., Ujjivan Small Finance Bank, Equitas Small Finance Bank, and Suryoday Small Finance Bank. Six financial parameters, named, Net Profit (NP), Return on Asset (ROA), Return on Capital Employed (ROCE), Current and Saving Account Ratio (CASA), Return on Equity (ROE) and revenue are considered as the dependent variables used to measure profitability and Gross Non-performing Asset (GNPA) and Net Non-performing Asset (NNPA) are the independent variables which represent NPA. The correlation is tested with Spearman’s rank correlation coefficient and t-test. The t-test result of Equities Bank shows that both GNPA and NNPA have correlation with all parameters except Net Profit. Bandhan Bank shows CASA, ROA ROE are correlated to GNPA, however, NNPA has correlation with CASA. The result of other two companies establish correlation of NPA to revenue, ROA, and CASA. This study concludes that GNPA has a significant impact on the profitability of the discussed microfinance companies, however the impact of NNPA is comparatively less significant than GNPA.